منابع مشابه
Properties and Biological Behaviour of Coomassie Blue.
The demonstration by Hamilton et al. (1948) and Werko et al. (1949) that the analysis of timeconcentration curves of intravenously injected Evans Blue (T-1824) gave estimates of the cardiac output in man that corresponded closely with those obtained by the direct Fick method stimulated a widespread use of the dye method. However, those properties of Evans Blue that led Dawson et al. (1920) to s...
متن کاملThe solubility of Coomassie blue protein-dye complexes.
Coomassie blue protein dye-binding assays are commonly used for quantitation of protein. They exploit the interaction between protein and Coomassie brilliant blue (CBB) G-250, the latter being solubilized in either ethanol/phosphoric acid (1) or perchloric acid (2). The Bradford assay (1) is the method of choice. Potential problems with these assays include non-linearity, a variable response to...
متن کاملAdsorption of Methylene Blue, Bromophenol Blue, and Coomassie Brilliant Blue by α-chitin nanoparticles
Expelling of dyestuff into water resource system causes major thread to the environment. Adsorption is the cost effective and potential method to remove the dyes from the effluents. Therefore, an attempt was made to study the adsorption of dyestuff (Methylene Blue (MB), Bromophenol Blue (BPB) and Coomassie Brilliant Blue (CBB)) by α-chitin nanoparticles (CNP) prepared from Penaeus monodon (Fabr...
متن کاملHeating greatly speeds Coomassie blue staining and destaining.
All loading buffers allowed the target amplification at 0.25 and 0.5 ×final concentration (Figure 2). However, at 0.5 × final concentration , there was a decrease in the amplification that was more pronounced than that observed with the 359 bp target depicted in Figure 1. After the 0.25 ×gel-loading buffers in the PCR mixture proved to be feasible even when the bacterial lysate was diluted 1000...
متن کاملRemoval of coomassie blue precipitates from polyacrylamide gels.
During staining of polyacrylamide gels, varying amounts of Coomassie blue may precipitate on the surface of the gels. This background obscures faint bands and is esthetically unappealing. The problem is worse when gels are stained for longer periods of time or when the stain has not been recently filtered. Rinsing a gel with methanol efficiently and quickly removes this background. Basically, a...
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ژورنال
عنوان ژورنال: Heart
سال: 1959
ISSN: 1355-6037
DOI: 10.1136/hrt.21.4.497